Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy

Sensors

Philipp Hörmann¹  Carsten Reuse¹  Karsten Hiller¹  Jana Hartmann²  Shinta Mariana²  AgusBudi Dharmawan²  Andreas Waag²  HutomoSuryo Wasisto²  Iqbal Syamsu²  Gregor Scholz²  JoanDaniel Prades³ 

[1] Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, 38106 Braunschweig, Germany;Institute of Semiconductor Technology (IHT), Technische Universität Braunschweig, 38106 Braunschweig, Germany;MIND-IN²UB, Department of Electronic and Biomedical Engineering, University of Barcelona, 08028 Barcelona, Spain;

关键词: lensless holographic microscopy;    LED;    Complementary Metal-Oxide Semiconductor (CMOS) image sensor;    cell culture;    cell imaging;    cell counting;   

DOI  :  10.3390/s19051234

来源: DOAJ

【 摘 要 】

Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.

【 授权许可】

Unknown