Variation of Metagenome From Feedstock to Digestate in Full-Scale Biogas Plants

Frontiers in Microbiology

Wei Fan²  Anqi Wang²  Sen Wang²  Fan Jiang²  Yan Zhang²  Hengchao Wang²  Hangwei Liu²  Yan Huang³  Yu Deng³  Qiang Li³  Hui Fan³  Lei Cheng³  Shichun Ma³ 

[1] Biogas Institute of Ministry of Agricultural and Rural Affairs, Chengdu, China;Guangdong Laboratory for Lingnan Modern Agriculture (Shenzhen Branch), Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China;Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agricultural and Rural Affairs, Chengdu, China;

关键词: metagenome;    anaerobic digestion;    feedstock;    full-scale biogas plants;    animal manure;   

DOI  :  10.3389/fmicb.2021.660225

来源: DOAJ

【 摘 要 】

Anaerobic digestion (AD) has been widely used to resolve the problem of organic wastes worldwide. Previous studies showed that the types of feedstock have a great influence on the AD microbiome, and a huge number of AD populations are migrated from upstream feedstocks. However, the changes of microbial compositions from feedstock to AD digestate are still less understood. We collected feedstock samples from 56 full-scale biogas plants, generated 1,716 Gb feedstock metagenomic data in total, and constructed the first comprehensive microbial gene catalog of feedstock containing 25.2 million genes. Our result indicated that the predominant phyla in feedstock are Firmicutes, Bacteroidetes, and Proteobacteria, which is similar to that in AD digestate, and the microbial diversity of feedstock samples is higher than that of AD digestate samples. In addition, the relative abundance of most genes involved in methanogenesis increase from feedstock to AD digestate. Besides, the amount of antibiotic resistance genes (ARGs) and pathogenic bacteria in AD are effectively reduced compared to feedstocks. This study provides a comprehensive microbial gene catalog of feedstock, and deepens the understanding of variation of microbial communities from feedstock to AD digestate of full-scale AD. The results also suggest the potential of AD to reduce the level of ARGs and pathogens in animal manure.

【 授权许可】

Unknown