Mutator-Derived lncRNA Landscape: A Novel Insight Into the Genomic Instability of Prostate Cancer

Frontiers in Oncology

Jiyan Liu¹  Wanjiang Li²  Qiang Wei³  Shi Qiu³  Jianzhong Ai³  Lu Yang³  Xiaonan Zheng⁴  Hang Xu⁴  Liansha Tang⁵  Wenbo He⁵ 

[1] Department of Biotherapy, West China Hospital, Sichuan University, Chengdu, China;Department of Radiology, West China Hospital of Sichuan University, Chengdu, China;Department of Urology, Institute of Urology, West China Hospital of Sichuan University, Chengdu, China;Institute of System Genetics, West China Hospital of Sichuan University, Chengdu, China;West China Medical School of Sichuan University, Chengdu, China;

关键词: long non-coding RNAs;    genome instability;    mutation phenotype;    prostate cancer;    biochemical recurrence;   

DOI  :  10.3389/fonc.2022.876531

来源: DOAJ

【 摘 要 】

BackgroundIncreasing evidence has emerged to reveal the correlation between genomic instability and long non-coding RNAs (lncRNAs). The genomic instability-derived lncRNA landscape of prostate cancer (PCa) and its critical clinical implications remain to be understood.MethodsPatients diagnosed with PCa were recruited from The Cancer Genome Atlas (TCGA) program. Genomic instability-associated lncRNAs were identified by a mutator hypothesis-originated calculative approach. A signature (GILncSig) was derived from genomic instability-associated lncRNAs to classify PCa patients into high-risk and low-risk groups. The biochemical recurrence (BCR) model of a genomic instability-derived lncRNA signature (GILncSig) was established by Cox regression and stratified analysis in the train set. Then its prognostic value and association with clinical features were verified by Kaplan–Meier (K-M) analysis and receiver operating characteristic (ROC) curve in the test set and the total patient set. The regulatory network of transcription factors (TFs) and lncRNAs was established to evaluate TF–lncRNA interactions.ResultsA total of 95 genomic instability-associated lncRNAs of PCa were identified. We constructed the GILncSig based on 10 lncRNAs with independent prognostic value. GILncSig separated patients into the high-risk (n = 121) group and the low-risk (n = 121) group in the train set. Patients with high GILncSig score suffered from more frequent BCR than those with low GILncSig score. The results were further validated in the test set, the whole TCGA cohort, and different subgroups stratified by age and Gleason score (GS). A high GILncSig risk score was significantly associated with a high mutation burden and a low critical gene expression (PTEN and CDK12) in PCa. The predictive performance of our BCR model based on GILncSig outperformed other existing BCR models of PCa based on lncRNAs. The GILncSig also showed a remarkable ability to predict BCR in the subgroup of patients with TP53 mutation or wild type. Transcription factors, such as FOXA1, JUND, and SRF, were found to participate in the regulation of lncRNAs with prognostic value.ConclusionIn summary, we developed a prognostic signature of BCR based on genomic instability-associated lncRNAs for PCa, which may provide new insights into the epigenetic mechanism of BCR.

【 授权许可】

Unknown