The horizontally-acquired response regulator SsrB drives a Salmonella lifestyle switch by relieving biofilm silencing

eLife

Saravanan Periasamy¹  Linda J Kenney²  Ricksen S Winardhi³  Stuti K Desai³  Yan Jie⁴  Michal M Dykas⁵ 

[1] Department of Physics, National University of Singapore, Singapore, Singapore;Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore, Singapore;Mechanobiology Institute, National University of Singapore, Singapore, Singapore;Nanoscience and Nanotechnology Institute, National University of Singapore, Singapore, Singapore;Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore;

关键词: biofilm;    salmonella typhimurium;    two-component regulatory system;    ssrA/B;    csgD/agfD;    H-NS;   

DOI  :  10.7554/eLife.10747

来源: DOAJ

【 摘 要 】

A common strategy by which bacterial pathogens reside in humans is by shifting from a virulent lifestyle, (systemic infection), to a dormant carrier state. Two major serovars of Salmonella enterica, Typhi and Typhimurium, have evolved a two-component regulatory system to exist inside Salmonella-containing vacuoles in the macrophage, as well as to persist as asymptomatic biofilms in the gallbladder. Here we present evidence that SsrB, a transcriptional regulator encoded on the SPI-2 pathogenicity-island, determines the switch between these two lifestyles by controlling ancestral and horizontally-acquired genes. In the acidic macrophage vacuole, the kinase SsrA phosphorylates SsrB, and SsrB~P relieves silencing of virulence genes and activates their transcription. In the absence of SsrA, unphosphorylated SsrB directs transcription of factors required for biofilm formation specifically by activating csgD (agfD), the master biofilm regulator by disrupting the silenced, H-NS-bound promoter. Anti-silencing mechanisms thus control the switch between opposing lifestyles.

【 授权许可】

Unknown