Platelet adhesion on commercially pure titanium plates in vitro I: effects of plasma components and involvement of the von Willebrand factor and fibronectin

International Journal of Implant Dentistry

Shotaro Takahashi¹  Koh Nakata²  Takao Watanabe³  Yutaka Kitamura⁴  Taisuke Watanabe⁵  Tomoyuki Kawase⁶  Kazushige Isobe⁷  Tetsuhiro Tsujino⁷ 

[1]

[2]Bioscience Medical Research Center, Niigata University Medical and Dental Hospital

[3]Department of Oral Science, Graduate School of Dentistry, Kanagawa Dental University

[4]Department of Oral and Maxillofacial Surgery, Matsumoto Dental University

[5]Division of Anatomy and Cell Biology of the Hard Tissue, Institute of Medicine and Dentistry, Niigata University

[6]Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University

[7]Tokyo Plastic Dental Society

关键词: Platelet;    Titanium;    Adhesion;    Fibronectin;    von Willebrand factor;    Fibrinogen;   

DOI  :  10.1186/s40729-019-0160-z

来源: DOAJ

【 摘 要 】

Abstract Background Platelet-rich plasma (PRP) is widely used in regenerative dentistry. Furthermore, it is often applied in the pretreatment of titanium implants to improve their surface bioaffinity and initial stability. However, effects of PRP application on implant surface at cellular and molecular levels remain poorly understood. Therefore, we examined platelet adhesion on commercially pure titanium (cp-Ti) plates, with a particular focus on fibrinogen (FGN), von Willebrand factor (vWF), and fibronectin (FN), in the presence or absence of plasma components. Methods Citrated blood samples were obtained from six healthy male volunteers, and pure-PRP (P-PRP) and pure platelet suspensions in phosphate-buffered saline (PBS) were prepared. Platelet adhesion on cp-Ti plate surface was evaluated by phalloidin staining and tetrazolium dye assay. Distribution of FGN, vWF, FN, albumin, CD62P, and CD63 was examined by immunocytochemical analysis. Results Platelets in PBS suspensions rapidly and time-dependently adhered to cp-Ti plate surface, but this adhesion was substantially disturbed by the presence of plasma components. FGN was most preferably adsorbed regardless of the presence or absence of plasma components, while vWF and FN showed greater accumulation on platelet adhesion area. Conclusions Although FGN is rapidly and abundantly adsorbed on cp-Ti plate surface, vWF and FN function as major platelet adhesion molecules in citrated blood samples. After pretreatment with P-PRP, however, platelets adhered to cp-Ti much less efficiently. Therefore, P-PRP pretreatment might not directly contribute to surface functionalization, initial stabilization, and osseointegration of machined or similar types of implants.

【 授权许可】

Unknown