Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

International Journal of Molecular Sciences

Paulina Kazimierczak¹  Agata Przekora¹  Grzegorz Kalisz²  Anna Sroka-Bartnicka²  Barbara Gieroba²  IzabelaS. Pieta³  Robert Nowakowski³  Marcin Pisarek³ 

[1] Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland;Department of Biopharmacy, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland;Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland;

关键词: biopolymers;    1,3-β-d-glucan;    vibrational spectroscopy;    X-ray photoelectron spectroscopy;    AFM microscopy;    biomaterials;   

DOI  :  10.3390/ijms21176154

来源: DOAJ

【 摘 要 】

In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen. Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes. X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity. Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices. In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM). Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration. This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.

【 授权许可】

Unknown