PD-1-stimulated T cell subsets are transcriptionally and functionally distinct

iScience

Shoiab Bukhari¹  Shalom Lerrer²  Anna S. Tocheva²  Kieran Adam²  Adam Mor² 

[1] Department of Genetics and Genomic Sciences, Ichan School of Medicine at Mount Sinai, New York, NY 10029, USA;Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY 10032, USA;

关键词: Immunology;    Cancer;    Transcriptomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Despite the obvious inhibitory outcome of PD-1 signaling, an additional series of functions are activated. We have observed that T cells stimulated through the T cell receptor (TCR) and PD-1 primarily do not proliferate; however, there is a population of cells that proliferates more than through TCR stimulation alone. In this study, we performed flow cytometry and RNA sequencing on individual populations of T cells and discovered that unlike naive T cells, which were inhibited following PD-1 ligation, T cells that proliferated more following PD-1 ligation were associated with effector and central memory phenotypes. We showed that these populations had different gene expression profiles following PD-1 ligation with PD-L1 compared to PD-L2. The presence of transcriptionally and functionally distinct T cell populations responsive to PD-1 ligation provides new insights into the biology of PD-1 and suggest the use of T cell subset-specific approaches to improve the clinical outcome of PD-1 blockade.

【 授权许可】

Unknown