Immediately implantable extracellular matrix-enriched osteoinductive hydrogel-laden 3D-printed scaffold for promoting vascularized bone regeneration in vivo

Materials & Design

Dong Nyoung Heo¹  Kyung Min Park²  Jung Bok Lee³  Seung Yeon Lee⁴  Il Keun Kwon⁵  Sang Jin Lee⁵  Jae Seo Lee⁶  Haram Nah⁶  Wan-Kyu Ko⁷  Kyu-Sun Choi⁸  Hyeong-joong Yi⁸  Sang-Hyun An⁹  Donghyun Lee⁹ 

[1] Corresponding authors at: Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea (D.N. Heo). Department of Neurosurgery, College of Medicine, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea (K.S. Choi).;Department of Bioengineering and Nano-bioengineering, Incheon National University, Incheon 22012, Republic of Korea;Department of Biological Science, Sookmyung Women’s University, Cheongpa-ro 47-gil 100, Yongsan-gu, Seoul 04310, Republic of Korea;Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea;Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea;Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea;Department of Neurosurgery, CHA University, CHA Bundang Medical Center, Gyeonggi-do 13496, Republic of Korea;Department of Neurosurgery, College of Medicine, Hanyang University, 222 Wangsimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea;Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu 41061, Republic of Korea;

关键词: Polycaprolactone;    Biomacromolecules;    3D printing;    3D hybrid model;    Bone tissue engineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

Reconstruction of patient-specific scaffolds to repair uniquely shaped bone defects remains a major clinical challenge in tissue engineering. Recently, three-dimensional (3D) printed scaffolds have received considerable attention as a promising technology for the rapid generation of custom shapes. However, synthetic polymers commonly used for 3D printing, such as polycaprolactone (PCL), lack the biological capacity to mimic native extracellular matrix functions to support cell growth and differentiation into desired tissues. We described the preparation and characterization of a 3D hybrid model for bone tissue engineering that comprises an extracellular matrix (ECM)-enriched hydrogel embedded in a PCL scaffold. The human bone marrow-derived mesenchymal stem cell–derived matrisome (BMTS) was utilized as a source of ECM-enriched biomacromolecules, and scaffold biocompatibility was evaluated in vitro using human bone marrow-derived mesenchymal stem cells (BM-MSCs). The 3D hybrid model exhibited excellent BM-MSC viability and osteogenic activity in vitro in both two-dimensional (2D) and 3D cultures. Furthermore, bone remodeling was evaluated by in vivo through a rat calvarial defect model; notably, the fabricated 3D hybrid model effectively enhanced vascularized bone regeneration. Therefore, this promising BMTS-based 3D hybrid model might serve as an excellent bone tissue-engineered scaffold for use in orthopedic applications.

【 授权许可】

Unknown