Phytocomplex Influences Antimicrobial and Health Properties of Concentrated Glycerine Macerates

Antibiotics

Stefania Garzoli¹  Maurizio Sanguinetti²  Margherita Gentile²  Margherita Cacaci²  Francesca Bugli²  Lorenzo Barbanti³  Paola Mattarelli³  Maura Di Vito³  Mattia Titubante⁴  Laura Micheli⁴  Claudia Mazzuca⁴  Alberto Vitali⁵ 

[1] Dipartimento di Chimica e Tecnologie del Farmaco, Università di Roma “Sapienza”, Piazzale Aldo Moro 5, 00100 Rome, Italy;Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Largo A. Gemelli 8, 00168 Rome, Italy;Dipartimento di Scienze e Tecnologie Agro-Alimentari, Università di Bologna, Viale G. Fanin 42, 40127 Bologna, Italy;Dipartimento di Scienze e Tecnologie Chimiche, Università degli Studi di Roma ‘Tor Vergata’, V.le della Ricerca Scientifica 1, 00133 Rome, Italy;Istituto di Scienze e Tecnologie Chimiche “G. Natta”—C.N.R., Largo F. Vito 1, 00168 Rome, Italy;

关键词: Alnus glutinosa;    Carpinus betulus;    Ficus carica;    IM-SPME/GC-MS system;    Ribes nigrum;   

DOI  :  10.3390/antibiotics9120858

来源: DOAJ

【 摘 要 】

The purpose of this study was to correlate the chemical composition of four commercial concentrated glycerine macerates (C-GMs), produced through the same extraction method, with their in vitro antimicrobial, antioxidant, and immunomodulatory properties, in order to evaluate their potential for healing upper airway diseases. C-GMs of Carpinus betulus (CB), Ficus carica (FC), Alnus glutinosa (AG) and Ribes nigrum (RN) were studied. The quality was evaluated using HPLC and IM-SPME/GC-MS systems; anti-oxidant and anti-microbial activities were assessed by the respective DPPH test, and micro-broth dilution test performed against 10 strains of Streptococcus pyogenes and 10 probiotic strains. ELISA and MTT tests were used to assess the immunomodulatory activity and the cytotoxicity of C-GMs, respectively. A significant correlation was found between the number of active compounds and the in vitro C-GMs effectiveness. Furthermore, the C-GMs of AG showed the best anti-microbial activity on pathological strains and, together with CB, the best anti-oxidant activity. The ELISA test exhibited a good immunomodulatory activity of RN. In vitro data support the integrated use of C-GMs of CB, AG, and RN in presence of airway diseases, and highlight the importance of standard procedures in cultivation, harvest and post-harvest treatments, as a premise for C-GMs with consistent characteristics.

【 授权许可】

Unknown