Electrochemical Characterization of Enzymatic Impedimetric Biosensor Destined to Detect Organochlorine Pesticide: the Diclofop-methyl

Sensors & Transducers

F. Benamia¹  Z. Djeghaba¹  S. Baali²  S. Zougar²  R. Kherrat²  N. Jaffrezic-Renault³ 

[1] Laboratory of Applied Organic Chemistry, Department of Chemistry, B. P. 12, Annaba, 23000, Algeria;Laboratory of Environmental Engineering, Department of Process Engineering,Faculty of Engineering Sciences, Badji Mokhtar University -Annaba, B. P. 12, Annaba, 23000, Algeria;University of Lyon, Laboratory of Analytical Sciences, UMR-CNRS 5180, Claude Bernard University,Lyon I, 43 Boulevard du 11 November 1918, 69622 Villeurbanne Cedex, France;

关键词: Impedimetric biosensor;    Condida Rugosa lipase;    Organochlorine pesticide;    Dichlofop-methyl;    Detection.;   

DOI  :  

来源: DOAJ

【 摘 要 】

Preservation of aquatic ecosystems requires early warning tools such as biosensors for in situ monitoring and continuously. Such tools can provide information about the nature of pollutants condition be designed to meet specific manner. This work proposes an impedimetric biosensor, using a bacterial enzyme (Condida Rugosa lipase) immobilized in an organic matrix composed of BSA (bovine serum albumin) and glutaraldehyde, capable of detecting organochlorine pesticides (Diclofop-methyl) state trace in aqueous media. Moreover, these measurements were carried out by varying a number of parameters characteristic of the system being studied in order to better define the role of the different elements involved in the development of the receiving part of the sensor as follows: Composition of the membrane, effect of temperature and the effect of the pH of the medium. The results show that the developed biosensor provides answers in an area of very low concentrations of the order of 5.99x10-14 - 5.88x10-3 g/L and a detection limit of 5.99x10-14 g/L for Diclofop-methyl. This biosensor detects the pollutant in a temperature range between 20 °C and 40 °C. We also studied the selectivity of the detection of the target substance in the presence of interfering with some heavy metals (cadmium, cobalt, zinc and lead) and other organophosphorus and organochlorines pesticide.

【 授权许可】

Unknown