Stable Deuterium Labeling of Histidine-Rich Lysine-Based Dendrimers

Molecules

Erkki Lahderanta¹  IrinaI. Tarasenko²  IgorM. Neelov³  MaximYu Ilyash³  MariyaE. Mikhailova⁴  NadezhdaN. Sheveleva⁴  DenisA. Markelov⁴  MikhailA. Vovk⁴ 

[1] Department of Physics, LUT University, Box 20, 53851 Lappeenranta, Finland;Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoi Prospect 31, V.O., 199004 Saint Petersburg, Russia;Saint Petersburg National Research University of Information Technologies, Mechanics and Optics (ITMO University), Kronverkskiy pr. 49, 197101 Saint Petersburg, Russia;Saint Petersburg State University, 7/9 Universitetskaya nab, 199034 Saint Petersburg, Russia;

关键词: peptide dendrimer;    deuterium labeling;    histidine;   

DOI  :  10.3390/molecules24132481

来源: DOAJ

【 摘 要 】

Peptide dendrimers, due to their biocompatibility and low toxicity, are highly promising candidates as nanocarriers for drugs and genes. The development of this kind of delivery system requires reliable monitoring of their metabolic and biological pathways. In this respect, hydrogen isotope labeling has tremendous importance, being a safe tool for detection of the labeled nanocarriers. In this work, we have synthesized new histidine-rich lysine-based dendrimers (Lys-2His dendrimer) with two linear histidine (His) residues in every inner segment. The presence of His residues has enabled us to perform controlled deuteration of Lys-2His dendrimers. The high deuteration degree (around 70%) does not practically change after redissolving the samples in H₂O and heating them at 40 °C, which indicates the isotopic label stability.

【 授权许可】

Unknown