Journal of Functional Foods | |
Chemical profile and inhibition of α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) activities by flavonoids from licorice (Glycyrrhiza uralensis Fisch) | |
Yuqing Zhao1  Ting Xiao2  Jingjing Lu3  Zhenghong Guo3  Wei Li4  Xiuli Niu4  | |
[1] Key Laboratory of Structure-based Drug Design and Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China;College of Pharmacy, Yanbian University, Yanji 133000, China;School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shengyang, China; | |
关键词: Licorice (Glycyrrhiza. uralensis Fisch); Flavonoids; α-glycosidase and protein tyrosine phosphatase 1B (PTP1B) inhibitory activities; Food ingredients; | |
DOI : | |
来源: DOAJ |
【 摘 要 】
Licorice, one of the oldest and most common herbal medicines, is used as a natural sweetener and flavoring agent in food products. This study aimed to investigate the inhibitory activity of flavonoids against α-glycosidase and protein tyrosine phosphatase 1B (PTP1B). Macroporous resins were used to isolate total flavonoids from licorice extract; the obtained total flavonoid content was >80%. The extracted flavonoids were then identified by HPLC-MS/MS, and 18 compounds were isolated. These compounds were further evaluated to determine α-glycosidase and PTP1B inhibitory activities. A total of 12 compounds demonstrated stronger activity than acarbose (IC50 = 5.42 ± 0.10 µg/mL); the compound 18 yielded higher IC50 (15.62 ± 0.20 µM) than NaVO4 (positive control; IC50 = 29.10 ± 0.20 µM). These results suggested that flavonoids from licorice are potential functional food ingredients that can be used to prevent and treat type 2 diabetes mellitus.
【 授权许可】
Unknown