Ethanol Extract of Campsis grandiflora Flower and Its Organic Acid Components Have Inhibitory Effects on Autoinducer Type 1 Quorum Sensing

Molecules

Gang Wang¹  Miao Wang¹  Leyu Wang²  Juanmei Zhang²  Fenghua Xu²  Lingling Yao² 

[1]Institute of Microbial Engineering, Laboratory of Bioresource and Applied Microbiology, School of Life Sciences, Hennan Univeristy, Kaifeng 475004, China

[2]School of Pharmaceutical, Henan University, Kaifeng 475004, China

关键词: quorum sensing;    Campsis grandiflora;    biofilm;    swarming;    malic acid;    succinic acid;   

DOI  :  10.3390/molecules25204727

来源: DOAJ

【 摘 要 】

Chinese herbs are a useful resource bank for natural drug development, and have attracted considerable attention to exploit quorum sensing inhibitors (QSIs). This study was designed to screen QSIs from raw Chinese herb materials. Of the 38 common herbs examined, the ethanol extract of Campsis grandiflora flower had the strongest QSI activity. The C. grandiflora flower ethanol extract (CFEE) was purified by HPD600, and the QSI activities were examined in further detail. CFEE inhibited violacein production of Chromobacterium violaceum 026 in a dose-dependent manner, and inhibit the swarming abilities of Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Furthermore, CFEE could inhibited biofilm formation and destroyed mature biofilms of E. coli K-12 and P. aeruginosa PAO1. The composition of CFEE was determined by UPLC-MS/MS to distinguish active QSI compounds, and 21 compounds were identified. In addition to gallic acid and caffeic acid, two organic acids, malic acid and succinic acid, were confirmed for the first time to have autoinducer type 1 QSI activities. Therefore, CFEE is a potential QSI that could be used as a novel antimicrobial agent and should be considered for medicinal development.

【 授权许可】

Unknown