Bacteria-Templated NiO Nanoparticles/Microstructure for an Enzymeless Glucose Sensor

International Journal of Molecular Sciences

Chien-Yen Chen¹  An-Cheng Sun²  Jong-Yuh Cherng³  Settu Vaidyanathan³ 

[1] Materials Science, Yuan Ze University. No. 135 Yuandong Road, Zhongli District, Taoyuan City 320, Taiwan;;Department of Chemical Engineering &Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Min-Hsiung, Chia-Yi 62102, Taiwan;

关键词: hollow cylinder NiO (HCNiO) nanostructure;    glassy carbon electrode (GCE);    non-enzymatic glucose sensor;    electrochemical sensing;    electrocatalysis;    amperometric sensors;   

DOI  :  10.3390/ijms17071104

来源: DOAJ

【 摘 要 】

The bacterial-induced hollow cylinder NiO (HCNiO) nanomaterial was utilized for the enzymeless (without GOx) detection of glucose in basic conditions. The determination of glucose in 0.05 M NaOH solution with high sensitivity was performed using cyclic voltammetry (CV) and amperometry (i–t). The fundamental electrochemical parameters were analyzed and the obtained values of diffusion coefficient (D), heterogeneous rate constant (ks), electroactive surface coverage (Г), and transfer coefficient (alpha-α) are 1.75 × 10−6 cm2/s, 57.65 M−1·s−1, 1.45 × 10−10 mol/cm2, and 0.52 respectively. The peak current of the i–t method shows two dynamic linear ranges of calibration curves 0.2 to 3.5 µM and 0.5 to 250 µM for the glucose electro-oxidation. The Ni2+/Ni3+ couple with the HCNiO electrode and the electrocatalytic properties were found to be sensitive to the glucose oxidation. The green chemistry of NiO preparation from bacteria and the high catalytic ability of the oxyhydroxide (NiOOH) is the good choice for the development of a glucose sensor. The best obtained sensitivity and limit of detection (LOD) for this sensor were 3978.9 µA mM−1·cm−2 and 0.9 µM, respectively.

【 授权许可】

Unknown