Metabolic cooperativity between Porphyromonas gingivalis and Treponema denticola

Journal of Oral Microbiology

Nada Slakeski¹  Eric C. Reynolds¹  Catherine A. Butler¹  Brigitte Hoffmann¹  Lin Xin Kin¹  Stuart G. Dashper¹ 

[1] The University of Melbourne;

关键词: chronic periodontitis;    porphyromonas gingivalis;    treponema denticola;    polymicrobial community;    dysbiosis;    metabolic cooperativity;   

DOI  :  10.1080/20002297.2020.1808750

来源: DOAJ

【 摘 要 】

Background  Porphyromonas gingivalis and Treponema denticola are proteolytic periodontopathogens that co-localize in polymicrobial subgingival plaque biofilms, display in vitro growth symbiosis and synergistic virulence in animal models of disease. These symbioses are underpinned by a range of metabolic interactions including cooperative hydrolysis of glycine-containing peptides to produce free glycine, which T. denticola uses as a major energy and carbon source. Objective  To characterize the P. gingivalis gene products essential for these interactions. Methods: The P. gingivalis transcriptome exposed to cell-free T. denticola conditioned medium was determined using RNA-seq. P. gingivalis proteases potentially involved in hydrolysis of glycine-containing peptides were identified using a bioinformatics approach. Results  One hundred and thirty-twogenes displayed differential expression, with the pattern of gene expression consistent with succinate cross-feeding from T. denticola to P. gingivalis and metabolic shifts in the P. gingivalis folate-mediated one carbon superpathway. Interestingly, no P. gingivalis proteases were significantly up-regulated. Three P. gingivalis proteases were identified as candidates and inactivated to determine their role in the release of free glycine. P. gingivalis PG0753 and PG1788 but not PG1605 are involved in the hydrolysis of glycine-containing peptides, making free glycine available for T. denticola utilization. Conclusion  Collectively these metabolic interactions help to partition resources and engage synergistic interactions between these two species.

【 授权许可】

Unknown