A Myocardial Slice Culture Model Reveals Alpha-1A-Adrenergic Receptor Signaling in the Human Heart

JACC: Basic to Translational Science

Teresa De Marco, MD¹  Philip M. Swigart, BS¹  Anthony J. Baker, PhD²  Patrick M. Cowley, PhD²  Bat-Erdene Myagmar, MD, PhD²  R. Croft Thomas, Jr., MD²  Paul C. Simpson, MD²  Megan D. Montgomery, PhD²  Abhishek Singh, MD, PhD² 

[1] Department of Medicine, University of California, San Francisco, San Francisco, California;Department of Medicine, VA Medical Center, San Francisco, California;

关键词: alpha-1-adrenergic;    cell signaling;    human models;   

DOI  :  10.1016/j.jacbts.2016.03.005

来源: DOAJ

【 摘 要 】

The authors used 52 nonfailing and failing human hearts to develop a simple, high throughput left ventricular myocardial slice model that is stable by ATP and viability assays for at least 3 days. The model supports studies of signaling, contraction, and viral transduction. They use the model to show for the first time that the alpha-1A-adrenergic receptor, which is present at very low abundance in the human myocardium, activates cardioprotective ERK with nanomolar EC50 in failing heart slices and stimulates a positive inotropic effect. This model should be useful for translational studies, to test whether molecules discovered in basic experiments are functional in the human heart.

【 授权许可】

Unknown