Exploring Gunnera tinctoria: From Nutritional and Anti-Tumoral Properties to Phytosome Development Following Structural Arrangement Based on Molecular Docking

Molecules

Samad N. Ebrahimi¹  Peyman Salehi¹  Faezeh Fathi¹  Ana I. G. Valadão²  Anabela S. G. Costa²  Rita C. Alves²  Nelson Andrade²  Maria Beatriz P. P. Oliveira²  Alireza Fathi³  Cláudia Silva⁴  Fátima Martel⁴ 

[1] Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran 1983969411, Iran;REQUIMTE/LAQV, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira No. 280, 4050-313 Porto, Portugal;Sana Technologists Segal Private Company (STM), Ashrafi Esfahani, Tehran 1469963811, Iran;Unit of Biochemistry, Department of Biomedicine, Faculty of Medicine of Porto, University of Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal;

关键词: bioactive compounds;    phytosome;    secondary layer;    molecular docking;    bilayer arrangement;   

DOI  :  10.3390/molecules26195935

来源: DOAJ

【 摘 要 】

Gunnera tinctoria, an underexplored invasive plant found in Azores, Portugal, was studied regarding its nutritional, antioxidant, and antitumoral properties. Higher antioxidant activity was found in baby leaves, followed by adult leaves and inflorescences. A phenolic fraction of the plant was enriched using adsorbent resin column chromatography (Diaion^TM HP20LX, and Relite EXA90). Antitumoral effects were observed with the enriched fractions in breast (MCF-7) and pancreatic (AsPC-1) cancer cell lines, being more pronounced in the latter. To improve protection and membrane absorption rates of phenolic compounds, nano-phytosomes and cholesterol-conjugated phytosomes coated with natural polymers were loaded with the enriched fraction. The particles were characterized, and their physiochemical properties were evaluated and compared. All samples presented anionic charge and nanometer size in relation to the inner layer and micrometer size regarding the external layers. In addition, the molecular arrangement of phenolics within both types of phytosomes were studied for the first time by molecular docking. Polarity and molecular size were key factors on the molecular arrangement of the lipid bilayer. In conclusion, G. tinctoria showed to be an interesting source of nutrients and phenolic compounds with anti-tumoral potential. Moreover, phytosome loading with these compounds can increase their stability and bioavailability having in view future applications.

【 授权许可】

Unknown