Photoacoustic and fluorescence lifetime imaging of diatoms

Photoacoustics

Alexey I. Salimon¹  Olga Yu. Kalinina²  Marina V. Novoselova³  Philipp V. Sapozhnikov⁴  Julijana Cvjetinovic⁴  Alexander M. Korsunsky⁴  Dmitry A. Gorin⁵  Alexey M. Yashchenok⁶  Evgeny A. Shirshin⁷ 

[1] Corresponding authors.;Institute of Spectroscopy of the Russian Academy of Sciences, 5 Fizicheskaya Str., Troitsk, Moscow, 108840, Russia;Center for Energy Science and Technology, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia;Center for Photonics and Quantum Materials, Skolkovo Institute of Science and Technology, 3 Nobelya Str., Moscow, 121205, Russia;Faculty of Geography, Lomonosov Moscow State University, 1 Leninskiye Gory, Moscow, 119991, Russia;Lomonosov Moscow State University, 1/2 Leninskiye Gory, Moscow, 119991, Russia;Shirshov Institute of Oceanology of Russian Academy of Sciences, 36 Nakhimovsky Prospekt, Moscow, 117997, Russia;

关键词: Diatoms;    Dynamic light scattering;    Absorbance;    Fluorescence spectroscopy;    Fluorescence lifetime imaging microscopy;    Photoacoustic imaging;   

DOI  :  

来源: DOAJ

【 摘 要 】

Photoacoustic and fluorescent methods are used intensely in biology and medicine. These approaches can also be used to investigate unicellular diatom algae that are extremely important for Earth’s ecology. They are enveloped within silica frustules (exoskeletons), which can be used in drug delivery systems. Here, we report for the first time the successful application of photoacoustic (PA) and fluorescent visualization of diatoms. Chlorophyll a and c and fucoxanthin were found likely to be responsible for the photoacoustic effect in diatoms. The PA signal was obtained from gel drops containing diatoms and was found to increase with the diatom concentration. The fluorescence lifetime of the diatom chromophores ranged from 0.5 to 2 ns. The dynamic light scattering, absorbance, and SEM characterization techniques were also applied. The results were considered in combination to elucidate the nature of the photoacoustic signal. Possible biotechnological applications are proposed for the remote photoacoustic monitoring of algae.

【 授权许可】

Unknown