Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase

Biomolecules

Lenka Luhová¹  Veronika Šmotková Kraiczová¹  Lucie Činčalová¹  Lucie Berčíková¹  Tereza Jedelská¹  Marek Petřivalský¹ 

[1] Department of Biochemistry, Faculty of Science, Palacký University, CZ-783 71 Olomouc, Czech Republic;

关键词: abiotic stress;    cadmium;    nitric oxide;    reactive oxygen species;    root growth;    S-nitrosation;    S-nitrosoglutathione reductase;    salinity;    Solanum habrochaites;    Solanum lycopersicum;   

DOI  :  10.3390/biom9090393

来源: DOAJ

【 摘 要 】

S-nitrosoglutathione reductase (GSNOR) exerts crucial roles in the homeostasis of nitric oxide (NO) and reactive nitrogen species (RNS) in plant cells through indirect control of S-nitrosation, an important protein post-translational modification in signaling pathways of NO. Using cultivated and wild tomato species, we studied GSNOR function in interactions of key enzymes of reactive oxygen species (ROS) metabolism with RNS mediated by protein S-nitrosation during tomato root growth and responses to salinity and cadmium. Application of a GSNOR inhibitor N6022 increased both NO and S-nitrosothiol levels and stimulated root growth in both genotypes. Moreover, N6022 treatment, as well as S-nitrosoglutathione (GSNO) application, caused intensive S-nitrosation of important enzymes of ROS metabolism, NADPH oxidase (NADPHox) and ascorbate peroxidase (APX). Under abiotic stress, activities of APX and NADPHox were modulated by S-nitrosation. Increased production of H₂O₂ and subsequent oxidative stress were observed in wild Solanum habrochaites, together with increased GSNOR activity and reduced S-nitrosothiols. An opposite effect occurred in cultivated S. lycopersicum, where reduced GSNOR activity and intensive S-nitrosation resulted in reduced ROS levels by abiotic stress. These data suggest stress-triggered disruption of ROS homeostasis, mediated by modulation of RNS and S-nitrosation of NADPHox and APX, underlies tomato root growth inhibition by salinity and cadmium stress.

【 授权许可】

Unknown