Metazoan evolution of glutamate receptors reveals unreported phylogenetic groups and divergent lineage-specific events

eLife

Jie Ji¹  Enrique Navas-Perez¹  Javier Luís¹  Pablo Fuentes-Prior¹  Nerea Roher²  Hector Escriva²  David Soto²  Esther Gratacòs-Batlle³  Àlex Bayés⁴  David Ramos-Vicente⁵  Rita Reig-Viader⁵  Jordi García-Fernández⁵  Demian Burguera⁵  Gemma Gou⁶ 

[1] Universitat Autònoma de Barcelona, Barcelona, Spain;Department of Genetics, School of Biology, Institut de Biomedicina, University of Barcelona, Barcelona, Spain;Institute of Biotechnology and Biomedicine, Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona, Barcelona, Spain;Molecular Bases of Disease, Biomedical Research Institute Sant Pau, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain;Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, Barcelona, Spain;Neurophysiology Laboratory, Department of Biomedicine, Medical School, August Pi i Sunyer Biomedical Research Institute, Institute of Neurosciences, Universitat de Barcelona, Barcelona, Spain;

关键词: phylogenetics;    ionotropic glutamate receptors;    metabotropic glutamate receptors;    electrophysiology;    gene expression;    amphioxus;   

DOI  :  10.7554/eLife.35774

来源: DOAJ

【 摘 要 】

Glutamate receptors are divided in two unrelated families: ionotropic (iGluR), driving synaptic transmission, and metabotropic (mGluR), which modulate synaptic strength. The present classification of GluRs is based on vertebrate proteins and has remained unchanged for over two decades. Here we report an exhaustive phylogenetic study of GluRs in metazoans. Importantly, we demonstrate that GluRs have followed different evolutionary histories in separated animal lineages. Our analysis reveals that the present organization of iGluRs into six classes does not capture the full complexity of their evolution. Instead, we propose an organization into four subfamilies and ten classes, four of which have never been previously described. Furthermore, we report a sister class to mGluR classes I-III, class IV. We show that many unreported proteins are expressed in the nervous system, and that new Epsilon receptors form functional ligand-gated ion channels. We propose an updated classification of glutamate receptors that includes our findings.

【 授权许可】

Unknown