Establishment of a Challenge Model for Sheeppox Virus Infection

Microorganisms

Mohamed El-Beskawy¹  Sahar Abd El Rahman²  Bernd Hoffmann³  Martin Beer³  Anne Pohlmann³  Janika Wolff³  Jacqueline King³ 

[1] Department of Animal Medicine, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51744, Egypt;Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt;Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, Insel Riems, D-17493 Greifswald, Germany;

关键词: capripox;    sheeppox;    SPPV;    field strain;    pathogenesis;    challenge model;   

DOI  :  10.3390/microorganisms8122001

来源: DOAJ

【 摘 要 】

Sheeppox virus (SPPV) together with goatpox virus and lumpy skin disease virus form the genus Capripoxvirus of the Poxviridae family. Due to their great economic importance and major impact on livelihood of small-scale farmers, OIE guidelines classify capripox viruses as notifiable diseases. In the present study, we examined pathogenesis of an Indian SPPV isolate and an Egyptian SPPV isolate in sheep. Three different infection routes were tested: (i) intravenous infection, (ii) intranasal infection and (iii) contact transmission between infected and naïve sheep. Clinical course, viremia and viral shedding as well as seroconversion were analyzed in order to establish a challenge model for SPPV infections that can be used in future vaccine studies. Next to in vivo characterization, both SPPV strains underwent next- and third-generation sequencing to obtain high quality full-length genomes for genetic characterization and comparison to already published SPPV sequences.

【 授权许可】

Unknown