Subcellular organization of UBE3A in human cerebral cortex

Molecular Autism

Matthew C. Judson¹  Benjamin D. Philpot¹  Richard J. Weinberg¹  Alain C. Burette²  Edward F. Chang³  William W. Seeley⁴  Alissa N. Li⁴ 

[1] Department of Cell Biology and Physiology and Neuroscience Center, University of North Carolina;Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill;Department of Neurological Surgery, University of California;Department of Neurology, University of California;

关键词: Angelman syndrome;    E6-AP;    Axon terminal;    Mitochondria;    Euchromatin;   

DOI  :  10.1186/s13229-018-0238-0

来源: DOAJ

【 摘 要 】

Abstract Background Loss of UBE3A causes Angelman syndrome, whereas excess UBE3A activity appears to increase the risk for autism. Despite this powerful association with neurodevelopmental disorders, there is still much to be learned about UBE3A, including its cellular and subcellular organization in the human brain. The issue is important, since UBE3A’s localization is integral to its function. Methods We used light and electron microscopic immunohistochemistry to study the cellular and subcellular distribution of UBE3A in the adult human cerebral cortex. Experiments were performed on multiple tissue sources, but our results focused on optimally preserved material, using surgically resected human temporal cortex of high ultrastructural quality from nine individuals. Results We demonstrate that UBE3A is expressed in both glutamatergic and GABAergic neurons, and to a lesser extent in glial cells. We find that UBE3A in neurons has a non-uniform subcellular distribution. In somata, UBE3A preferentially concentrates in euchromatin-rich domains within the nucleus. Electron microscopy reveals that labeling concentrates in the head and neck of dendritic spines and is excluded from the PSD. Strongest labeling within the neuropil was found in axon terminals. Conclusions By highlighting the subcellular compartments in which UBE3A is likely to function in the human neocortex, our data provide insight into the diverse functional capacities of this E3 ligase. These anatomical data may help to elucidate the role of UBE3A in Angelman syndrome and autism spectrum disorder.

【 授权许可】

Unknown