Cells | |
TILLING-by-Sequencing+ Reveals the Role of Novel Fatty Acid Desaturases (GmFAD2-2s) in Increasing Soybean Seed Oleic Acid Content | |
Amer AbuGhazaleh1  MohamedG. Embaby1  Tarek Hewezi2  ValériaStefania Lopes-Caitar2  Naoufal Lakhssassi3  Khalid Meksem3  MalloryA. Cullen3  Dounya Knizia3  Zhou Zhou3  Abdelhalim El Baze3  Oussama Badad3  TriD. Vuong4  HenryT. Nguyen4  Pengyin Chen4  Aicha Lakhssassi5  Jonas Meksem6  | |
[1] Department of Animal Science, Food, and Nutrition, Southern Illinois University, Carbondale, IL 62901, USA;Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA;Department of Plant, Soil and Agricultural Systems, Southern Illinois University, Carbondale, IL 62901, USA;Division of Plant Sciences, University of Missouri, Columbia, MO 65211, USA;Faculty of Sciences and Technologies, University of Lorraine, 54506 Nancy, France;Trinity College of Arts and Sciences, Duke University, Durham, NC 27708, USA; | |
关键词: high oleic acid; alternative fatty acid pathway; GmFAD2-2 subfamily; TILLING-by-Sequencing+; subfunctionalization; neofunctionalization; | |
DOI : 10.3390/cells10051245 | |
来源: DOAJ |
【 摘 要 】
Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.
【 授权许可】
Unknown