Genome-Wide Analysis of Coding and Long Non-Coding RNAs Involved in Cuticular Wax Biosynthesis in Cabbage (Brassica oleracea L. var. capitata)

International Journal of Molecular Sciences

Jinxiu Chen¹  Xiaowei Zhu¹  Yunying Ren¹  Tianyue Bo¹  Xiang Tai¹ 

[1] Horticulture Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201403, China;

关键词: cabbage;    nwgl phenotype;    fine mapping;    cuticular wax biosynthesis;    RNA-seq;    differential expressed genes;    lncRNAs;   

DOI  :  10.3390/ijms20112820

来源: DOAJ

【 摘 要 】

Cuticular wax is a mixture of very long chain fatty acids (VLCFAs) and their derivatives, which determines vital roles for plant growth. In cabbage, the cuticular wax content of leaf blades is an important trait influencing morphological features of the head. Understanding the molecular basis of cuticular wax biosynthesis can help breeders develop high quality cabbage varieties. Here, we characterize a cabbage non-wax glossy (nwgl) plant, which exhibits glossy green phenotype. Cryo-scanning electron microscope analysis showed abnormal wax crystals on the leaf surfaces of nwgl plants. Cuticular wax composition analyzed by GC-MS displayed severely decreased in total wax loads, and individual wax components in nwgl leaves. We delimited the NWGL locus into a 99-kb interval between the at004 marker and the end of chromosome C08 through fine mapping. By high-throughput RNA sequencing, we identified 1247 differentially expressed genes (DEGs) and 148 differentially expressed lncRNAs in nwgl leaves relative to the wild-type. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the DEGs and cis-regulated target genes for differentially expressed lncRNAs were significantly enriched in wax and lipid biosynthetic or metabolic processes. Our results provide the novel foundation to explore the complex molecular basis of cuticular wax biosynthesis.

【 授权许可】

Unknown