eLife | |
Direct observation of frequency modulated transcription in single cells using light activation | |
Xiuhau Meng1  David S Lawrence2  Liang Sun3  Robert H Singer3  Christoph Fritzsch4  Daniel R Larson5  | |
[1] Department of Pharmacology, University of North Carolina, Chapel Hill, United States;Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, United States;Department of Chemistry, Division of Chemical Biology and Medicinal Chemistry, University of North Carolina, Chapel Hill, United States;Institute of Molecular Biology, Johannes Gutenberg University of Mainz, Mainz, Germany;Laboratory of Receptor Biology and Gene Expression, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, United States; | |
关键词: transcription factor; gene expression; single-molecule; fluorescence; | |
DOI : 10.7554/eLife.00750 | |
来源: DOAJ |
【 摘 要 】
Single-cell analysis has revealed that transcription is dynamic and stochastic, but tools are lacking that can determine the mechanism operating at a single gene. Here we utilize single-molecule observations of RNA in fixed and living cells to develop a single-cell model of steroid-receptor mediated gene activation. We determine that steroids drive mRNA synthesis by frequency modulation of transcription. This digital behavior in single cells gives rise to the well-known analog dose response across the population. To test this model, we developed a light-activation technology to turn on a single steroid-responsive gene and follow dynamic synthesis of RNA from the activated locus.
【 授权许可】
Unknown