Comparative growth potential of thermophilic amylolytic Bacillus sp. on unconventional media food wastes and its industrial application

Saudi Journal of Biological Sciences

Ameena A. AL-Surhanee¹  Samina Qamer²  Maryam M. Alnoman³  Ali Hussain⁴  Ghullam Mustafa⁴  Shahzad Ali⁴  Tahira Younis⁵  Faiza Jabeen⁵  Fareeha Saleh⁵  Ahmad Ali⁶  Muhammad Rashid⁷ 

[1] Corresponding author.;Biology Department Faculty of Science, Taibah University, Yanbu, Saudi Arabia;Biology Department, College of Science, Jouf University, Sakaka 2014, Saudi Arabia;Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan;Department of Zoology, Government College University Faisalabad, Pakistan;Department of Zoology, The Islamia University of Bahawalpur, Pakistan;Faculty of Fisheries and Wildlife, University of Veterinary and Animal Sciences, Lahore, Pakistan;

关键词: Food wastes;    Thermophilic;    Bacillus sp.;    Thermostable amylases;   

DOI  :  

来源: DOAJ

【 摘 要 】

Amylases take part with vital role in industries such as food, fermentation; starch processing, textile and paper etc. Increasing amylases demand, high nutrient expenditure and environmental pollution have forced to utilize agro-industrial residues as a low-cost feedstock for enzyme production. In present study, three soil samples were collected from agro-industrial waste dumping areas in District Faisalabad. Ten thermophilic bacterial isolates were separated at 55 °C on the basis of colonial morphology, three isolates (F6, F11, F17) showed prominent zone of clearance applying iodine test on starch agar plates. Bacterial isolate F-11 showed highest amylase activity with DNS method and molecularly identified through 16S RNA sequencing as Bacillus sp. with Accession number MH917294. Four unconventional food wastes (banana, lemon, mango and potato) pretreated with 0.8% sulphuric acid concentrations taking 1000 g/L weight released the highest sugars contents and phenolic components. Maximum amylase activity i.e. 29.23 mg/ml was achieved in mango waste at, 40 °C, with pH 6.0 and 0.17% nitrogenous source adding 8% inoculum size (2 days old) using Response Surface Methodology (RSM) for optimization. Crude amylase confirmed its efficiency in starch hydrolysis that suggested it as potential candidate for application in starch industries.

【 授权许可】

Unknown