Increased ApoE Expression in Follicular Fluid and the ApoE Genotype Are Associated With Endometriosis in Chinese Women

Frontiers in Endocrinology

Xin Du¹  Yun-Xia Cao³  Dong-Mei Ji³  Meng-Yao Wang³  Ya-Jing Liu³  Ling-Ge Shi³  Si-Min Ding³  Fen Xing³  Zhao-Lian Wei³  Jin-Ping Qiao⁴  Yun-He Xia⁵  An Wang⁵  Yu-Hang Zhao⁵  Kai Zong⁹ 

[1] 901st Hospital of People’s Liberation Army (PLA) Joint Logistic Support Force, Hefei, China;Anhui Province Key Laboratory of Reproductive Health and Genetics, Anhui Medical University, Hefei, China;Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, Hefei, China;Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, China;First Clinical Medical College, Anhui Medical University, Hefei, China;Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, Hefei, China;National Health Commission (NHC) Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Anhui Medical University, Hefei, China;Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, China;Technical Center of Hefei Customs District, Hefei, China;

关键词: apolipoprotein E;    endometriosis;    follicular fluid;    genotype;    multifactor prediction model;   

DOI  :  10.3389/fendo.2021.779183

来源: DOAJ

【 摘 要 】

More than 10% of women suffer from endometriosis (EMT) during their reproductive years. EMT can cause pain and infertility and requires further study from multiple perspectives. Previous reports have indicated that an increase inapolipoprotein E (ApoE) may be associated with a lower number of retrieved mature oocytes in older women, and an association between ApoE and spontaneous pregnancy loss may exist in patients with EMT. The purpose of this study was to investigate the existence of an increase in ApoE in follicular fluid (FF) and the possible relationship between ApoE and EMT in Chinese women. In the current study, 217 Chinese women (111 control subjects and 106 EMT patients) were included. The ApoE genotypes were identified by Sanger sequencing. We found that ApoE expression in FF was higher in patients with EMT than in the control group. In addition, a significant difference in ApoE4 carriers (ϵ3/ϵ4, ϵ4/ϵ4) was found between the control subjects and the patients with EMT. Furthermore, a nonparametric test revealed significant differences in the numbers of blastocysts and high-quality blastocysts, but not the hormone levels of FSH, LH, and E2, between the two groups. We also established a multifactor (BMI, high-quality blastocysts, and ϵ4) prediction model with good sensitivity for identifying patients who may suffer from EMT. Our results demonstrate that ApoE expression in FF is increased in EMT, the ApoE-ϵ4 allele is significantly linked to EMT, and a combined analysis of three factors (BMI, high-quality blastocysts, and ϵ4) could be used as a predictor of EMT.

【 授权许可】

Unknown