Observations of membrane fusion in a liposome dispersion: the missing fusion intermediate? [v2; ref status: indexed, http://f1000r.es/56t]

F1000Research

Marianna Foldvari¹ 

[1] School of Pharmacy, Waterloo Institute of Nanotechnology, University of Waterloo, Waterloo, ON N2L 3G1, Canada;

关键词: Membranes & Sorting;   

DOI  :  10.12688/f1000research.6003.2

来源: DOAJ

【 摘 要 】

Early intermediate structures of liposome-liposome fusion events were captured by freeze-fracture electron microscopic (EM) technique. The images show the morphology of the fusion interface at several different stages of the fusion event. One of the intermediates was captured at a serendipitous stage of two vesicles’ membranes (both leaflets) merging and their contents starting to intermix clearly showing the fusion interface with a previously unseen fusion rim. From the morphological information a hypothetical sequence of the fusion event and corresponding lipid structural arrangements are described.

【 授权许可】

Unknown