Glycosylation of Ganoderic Acid G by Bacillus Glycosyltransferases

International Journal of Molecular Sciences

Tzi-Yuan Wang¹  Yun-Rong Zhang²  Te-Sheng Chang²  Hsiou-Yu Ding³  Jiumn-Yih Wu⁴ 

[1] Biodiversity Research Center, Academia Sinica, Taipei 11529, Taiwan;Department of Biological Sciences and Technology, National University of Tainan, Tainan 70005, Taiwan;Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, No. 60, Erh-Jen Rd., Sec. 1, Jen-Te District, Tainan 71710, Taiwan;Department of Food Science, National Quemoy University, Kinmen County 892, Taiwan;

关键词: Bacillus;    Ganoderma lucidum;    glycosyltransferase;    saponin;    triterpenoid;   

DOI  :  10.3390/ijms22189744

来源: DOAJ

【 摘 要 】

Ganoderma lucidum is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous Ganoderma triterpenoids have already been identified, rare Ganoderma triterpenoid saponins were recently discovered. To create novel Ganoderma saponins, ganoderic acid G (GAG) was selected for biotransformation using four Bacillus glycosyltransferases (GTs) including BtGT_16345 from the Bacillus thuringiensis GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the Bacillus subtilis ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-o-β-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-o-β-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four Bacillus GTs highlights the possibility of novel Ganoderma triterpenoid saponin production in the future.

【 授权许可】

Unknown