Comparative analysis of draft genome assemblies developed from whole genome sequences of two Hyaloperonospora brassicae isolate samples differing in field virulence on Brassica napus

Biotechnology Reports

Solomon Maina¹  Javed Akhatar²  Meenakshi Mittal²  Martin J. Barbetti³  Surinder S. Banga³  Ming Pei You³ 

[1] Corresponding authors.;Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004 Punjab, India;School of Agriculture and Environment and the UWA Institute of Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.;

关键词: Downy mildew;    Hyaloperonospora brassicae;    Genome sequence;    Crucifers;    Brassicaceae;    Rapeseed;   

DOI  :  

来源: DOAJ

【 摘 要 】

Hyaloperonospora brassicae causes downy mildew, a major disease of Brassicaceae species. We sequenced the genomes of two H. brassicae isolates of high (Sample B) and low (Sample C) virulence. Sequencing reads were first assembled de novo with software's SOAPdenovo2, ABySS V2.1 and Velvet V1.1 and later combined to create meta-assemblies with genome sizes of 72.762 and 76.950Mb and predicted gene densities of 1628 and 1644 /Mb, respectively. We could annotate 12.255 and 13,030 genes with high proportions (91-92%) of complete BUSCOs for Sample B and C, respectively. Comparative analysis revealed conserved and varied molecular machinery underlying the physiological specialisation and infection capabilities. BLAST analysis against PHI gene database suggested a relatively higher loss of genes for virulence and pathogenicity in Sample C compared to Sample B, reflecting pathogen evolution through differential rates of mutation and selection. These studies will enable identification and monitoring of H. brassicae virulence factors prevailing in-field.

【 授权许可】

Unknown