Drug Resistance Determinants in Clinical Isolates of Enterococcus faecalis in Bangladesh: Identification of Oxazolidinone Resistance Gene optrA in ST59 and ST902 Lineages

Microorganisms

Noriko Urushibara¹  Ayako Sumi¹  MeijiSoe Aung¹  Mitsuyo Kawaguchiya¹  Nobumichi Kobayashi¹  Arup Islam²  Salma Ahmed²  Anindita Paul²  Sangjukta Roy²  Nazia Haque²  EmilyRahman Khan²  Chand Sultana²  Sahida Abedin²  MuhammadAkram Hossain³  TridipKanti Barman⁴  ShyamalKumar Paul⁵ 

[1] Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan;Department of Microbiology, Mymensingh Medical College, Mymensingh 2200, Bangladesh;Imperial Hospital Ltd. Chittagong, Chittagong 4202, Bangladesh;Medicine Unit, Mymensingh Medical College Hospital, Mymensingh 2200, Bangladesh;Netrokona Medical College, Netrokona 2400, Bangladesh;

关键词: Enterococcus faecalis;    molecular epidemiology;    drug resistance;    optrA;    ST;   

DOI  :  10.3390/microorganisms8081240

来源: DOAJ

【 摘 要 】

Enterococcus faecalis is one of the major causes of urinary tract infection, showing acquired resistance to various classes of antimicrobials. The objective of this study was to determine the prevalence of drug resistance and its genetic determinants for E. faecalis clinical isolates in north-central Bangladesh. Among a total of 210 E. faecalis isolates, isolated from urine, the resistance rates to erythromycin, levofloxacin, and gentamicin (high level) were 85.2, 45.7, and 11.4%, respectively, while no isolates were resistant to ampicillin, vancomycin and teicoplanin. The most prevalent resistance gene was erm(B) (97%), and any of the four genes encoding aminoglycoside modifying enzyme (AME) were detected in 99 isolates (47%). The AME gene aac(6′)-Ie-aph(2”)-Ia was detected in 46 isolates (21.9%) and was diverse in terms of IS256-flanking patterns, which were associated with resistance level to gentamicin. Tetracycline resistance was ascribable to tet(M) (61%) and tet(L) (38%), and mutations in the quinolone resistance-determining region of both GyrA and ParC were identified in 44% of isolates. Five isolates (2.4%) exhibited non-susceptibility to linezolide (MIC, 4 μg/mL), and harbored the oxazolidinone resistance gene optrA, which was located in a novel genetic cluster containing the phenicol exporter gene fexA. The optrA-positive isolates belonged to ST59, ST902, and ST917 (CC59), while common lineages of other multiple drug-resistant isolates were ST6, ST28, CC16, and CC116. The present study first revealed the prevalence of drug resistance determinants of E. faecalis and their genetic profiles in Bangladesh.

【 授权许可】

Unknown