N6-methyladenosine methyltransferase KIAA1429 elevates colorectal cancer aerobic glycolysis via HK2-dependent manner

Bioengineered

Liang He¹  Yingkai Wang²  Ying Li²  Fan Zhang²  Yan Tan³ 

[1] Department of Gastrocolorectal Surgery, The First Hospital of Jilin University, Changchun, China;Department of Gastroenterology, The First Hospital of Jilin University, Changchun, China;Tumor Comprehensive Treatment Center, Jilin Provincial People's Hospital, Changchun, China;

关键词: N6-methyladenosine;    colorectal cancer;    KIAA1429;    HK2;    aerobic glycolysis;   

DOI  :  10.1080/21655979.2022.2065952

来源: DOAJ

【 摘 要 】

Emerging evidence has emphasized the critical roles played by N6-methyladenosine RNA (m6A) modification in colorectal carcinoma (CRC) initiation and progression. However, the roles and mechanism of m6A and KIAA1429 in CRC progression require further clarification. Here, our research aimed to investigate the functions of KIAA1429 in CRC tumorigenesis. Results indicated that KIAA1429 up-regulation closely correlated to the poor prognosis of CRC patients. Bio-functional assays demonstrated that KIAA1429 promoted the aerobic glycolysis, including glucose uptake, lactate production, ATP generation and extracellular acidification rate (ECAR). Mechanistically, KIAA1429 positively up-regulated HK2 level via increasing its mRNA stability by binding the m6A site of HK2 mRNA via m6A-independent manner. Collectively, our work indicates that KIAA1429 has the potential to promote CRC carcinogenesis by targeting HK2 via m6A-independent manner, providing insight into the critical roles of m6A in CRC.

【 授权许可】

Unknown