Antimicrobial Activity and Synergy Investigation of Hypericum scabrum Essential Oil with Antifungal Drugs

Molecules

Naïm Ouaini¹  Marc El Beyrouthy¹  Sara Vitalini²  Marcello Iriti²  Véronique Eparvier³  Didier Stien³  Layal Fahed³ 

[1] Department of Agricultural Sciences, Holy Spirit University of Kaslik, Jounieh 1200, Lebanon;Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, 20133 Milan, Italy;UPR 2301, Institut de Chimie des Substances Naturelles, CNRS, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette, France;

关键词: Hypericum scabrum;    essential oil;    chemical composition;    antimicrobial activity;    synergy;   

DOI  :  10.3390/molecules26216545

来源: DOAJ

【 摘 要 】

The chemical composition of Lebanese Hypericum scabrum essential oil (EO) was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GG-MS). Its antimicrobial activity was evaluated by determining its minimal inhibitory concentrations (MICs) against a Gram-negative and a Gram-positive bacterium, one yeast, and five dermatophytes. H. scabrum EO was most active on filamentous fungi (MIC values of 32–64 µg/mL). Synergy within the oil was investigated by testing each of the following major components on Trichophyton rubrum: α-pinene, limonene, myrcene, β-pinene and nonane, as well as a reconstructed EO. The antifungal activity of the natural oil could not be reached, meaning that its activity might be due, in part, to minor constituent(s). The interactions between H. scabrum EO and commercially available antifungals were assessed by the checkerboard test. A synergistic effect was revealed in the combination of the EO with amphotericin B.

【 授权许可】

Unknown