Uric acid aggravates myocardial ischemia–reperfusion injury via ROS/NLRP3 pyroptosis pathway

Biomedicine & Pharmacotherapy

JianLong Sheng¹  Fei He²  BangLong Xu²  ShiChun Shen²  Cheng Cheng² 

[1] Corresponding author at: Department of Cardiology, The Second Affiliated Hospital of Anhui Medical University, No. 678 FuRong Road, Hefei, Anhui Province, 230601, China.;Department of Cardiology, The Second Affiliated Hospital of Anhui Medical University, No. 678 FuRong Road, Hefei, Anhui Province, 230601, China;

关键词: Uric acid;    Myocardial ischemia–reperfusion;    Reactive oxygen species;    NLRP3 inflammasome;    Pyroptosis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: The NOD–like receptor pyrin domain–containing protein 3 (NLRP3) inflammasome activation–mediated pyroptosis pathway has been linked to myocardial ischemia–reperfusion (MI/R) injury. This study explored whether uric acid (UA) aggravates MI/R injury through NLRP3 inflammasome–mediated pyroptosis. Methods: In vivo, a mouse MI/R model was established by ligating the left coronary artery, and a mouse hyperuricemia model was created by intraperitoneal injection of potassium oxonate (PO). Then, the myocardial infarction (MI) size; terminal deoxynucleotidyl transferase dUTP nick end–labeling (TUNEL) immunofluorescence; and serum levels of lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK–MB), and UA, as well as the expression level of pyroptosis–related protein and caspase–3 in heart tissues, were measured. Separately, primary mouse cardiomyocytes were cultured in vitro to create a hypoxia/reoxygenation (H/R) model. We then compared cardiomyocytes viability, TUNEL immunofluorescence, and the levels of LDH, reactive oxygen species (ROS), and pyroptosis–related protein and caspase–3 in cardiomyocytes. Results: In vivo, the MI area, levels of CK–MB and LDH, rate of cell death, and pyroptosis–related protein and the expression of caspase–3 were significantly higher in the MI/R group than in the sham group, and high UA levels worsened these changes. In vitro, cardiomyocytes viability was significantly downregulated, and the levels of ROS, LDH, pyroptosis–related protein, caspase–3, and the rate of cardiomyocyte death were significantly higher in the H/R + UA group compared with the HR group. Administration of an NLRP3 inflammasome inhibitor and ROS scavenger reversed these effects. Conclusion: UA aggravates MI/R–induced activation of the NLRP3 inflammatory cascade and pyroptosis by promoting ROS generation, while inflammasome inhibitors and ROS scavengers partly reverse the injury.

【 授权许可】

Unknown