期刊论文详细信息
Saudi Journal of Biological Sciences
Toxicological interaction between tobacco smoke toxicants cadmium and nicotine: An in-vitro investigation
Mohammad A. Altamimi1  Nemat Ali2  Muneeb U. Rehman3  Fawaz Essa Alanazi4  Faisal Imam4  Wajhul Qamar5 
[1] Corresponding author.;Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;Department of Pharmacology and Toxicology, and Central Laboratory, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;
关键词: Cadmium;    Smoking;    Nicotine;    Lung toxicity;    A549;   
DOI  :  
来源: DOAJ
【 摘 要 】

Cigarettes and other tobacco products are used to obtain nicotine that is responsible for their stimulating effects. However, a lot of other organic and inorganic chemicals are also released along with nicotine. Cadmium (Cd) is one of the several heavy metals that are health hazards and is one of the inorganic elements released in tobacco smoke. The in-vitro investigation focused on exploring the effects of nicotine hydrogen tartrate (NHT) and cadmium (Cd) and their toxic interactions in the A549 cell line. In cell viability assay NHT exhibited its IC50 at 11.71 mM concentration, and the IC50 of Cd was found to be 83 µM after a 24 h exposure. Toxic effects of NHT (5 mM and 10 mM), Cd (50 µM and 100 µM), and their combination were also investigated by flowcytometry. The investigation included apoptotic and necrotic events, the effect on different cell cycle phases, and generation of reactive oxygen species by NHT, Cd, and their combination of different concentrations. Data reveal evident toxic effects of NHT, Cd, and NHT + Cd. It also indicates that the toxic interaction of NHT and Cd is not additive and appears to be minimal when compared with NHT or Cd exposures alone.

【 授权许可】

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