Circulating Levels of Pro-Neurotensin and Its Relationship with Nonalcoholic Steatohepatitis and Hepatic Lipid Metabolism

Metabolites

Cristóbal Richart¹  Carmen Aguilar¹  Laia Bertran¹  Salomé Martínez²  Jessica Binetti³  Teresa Auguet³  David Riesco³  Beatriz Villar³  Monica Real³  Daniel Del Castillo⁴  Fàtima Sabench⁴  Marta París⁴ 

[1] Grup d’Estudi de Malalties Metabòliques associades a Insulino Resistència (GEMMAIR)–AGAUR, Departament de Medicina i Cirurgia, Institut d’Investigació Sanitària Pere Virgili (IISPV), Universitat Rovira i Virgili, 4, 43007 Tarragona, Spain;Servei Anatomia Patològica, Hospital Universitari Joan XXIII Tarragona, 4, 43007 Tarragona, Spain;Servei Medicina Interna, Hospital Universitari Joan XXIII, Universitat Rovira i Virgili, 4, 43007 Tarragona, Spain;Servei de Cirurgia, Hospital Sant Joan de Reus, Institut d’Investigació Sanitària Pere Virgili. Avinguda, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili, 2, 43204 Reus, Spain;

关键词: pro-neurotensin;    neurotensin;    nonalcoholic fatty liver disease;    nonalcoholic steatohepatitis;    lipid metabolism;   

DOI  :  10.3390/metabo11060373

来源: DOAJ

【 摘 要 】

Recent studies suggest a link between pro-neurotensin (pro-NT) and nonalcoholic fatty liver disease (NAFLD), but the published data are conflicting. Thus, we aimed to analyze pro-NT levels in women with morbid obesity (MO) and NAFLD to investigate if this molecule is involved in NAFLD and liver lipid metabolism. Plasma levels of pro-NT were determined in 56 subjects with MO and 18 with normal weight (NW). All patients with MO were subclassified according to their liver histology into the normal liver (NL, n = 20) and NAFLD (n = 36) groups. The NAFLD group had 17 subjects with simple steatosis (SS) and 19 with nonalcoholic steatohepatitis (NASH). We used a chemiluminescence sandwich immunoassay to quantify pro-NT in plasma and RT-qPCR to evaluate the hepatic mRNA levels of several lipid metabolism-related genes. We reported that pro-NT levels were significantly higher in MO with NAFLD than in MO without NAFLD. Additionally, pro-NT levels were higher in NASH patients than in NL. The hepatic expression of lipid metabolism-related genes was found to be altered in NAFLD, as previously reported. Additionally, although pro-NT levels correlated with LDL, there was no association with the main lipid metabolism-related genes. These findings suggest that pro-NT could be related to NAFLD progression.

【 授权许可】

Unknown