期刊论文详细信息
Cell Communication and Signaling
mTORC1 and mTORC2 are differentially engaged in the development of laser-induced CNV
Tae Kwann Park1  Jin Young Yang1  Sanjar Batirovich Madrakhimov1  Hun Soo Chang2  Sang Joon Jung3  Seung Kwan Nah3  Dong Hyuck Ahn4  Ha Yan Park4 
[1] Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang Graduate School, Bucheon Hospital;Department of Medical Bioscience, Graduated School, Soonchunhyang University;Department of Ophthalmology, College of Medicine, Soonchunhyang University;Laboratory for Translational Research on Retinal and Macular Degeneration, Soonchunhyang University Hospital Bucheon;
关键词: Age-related macular degeneration;    Choroidal neovascularization;    Sirolimus (rapamycin);    mTORC1;    mTORC2;   
DOI  :  10.1186/s12964-019-0380-0
来源: DOAJ
【 摘 要 】

Abstract Background The mechanistic target of rapamycin (mTOR) pathway is a potential target to inhibit pathologic processes in choroidal neovascularization. However, the exact role of mTOR signaling in the development of CNV remains obscure. In this study, we assessed the role of mTORC1 and mTORC2 as well as the effect of rapamycin (sirolimus) on choroidal neovascularization (CNV) in a laser-induced mouse model. Methods In experiment A, we observed the natural course of CNV development and the dynamics of mTOR-related proteins during the 12 days after the laser injury. The expression of mTOR-related proteins was evaluated using Western blot (WB). Cryosections of CNV-induced mice were immunostained for the visualization of the vascular and extravascular components of the CNV. Experiment B was performed to confirm the critical period of mTOR signaling in the development of laser-induced CNV, we administered rapamycin before and/or during the active period of mTOR complexes. WB and immunofluorescence staining was performed to evaluate the mode of action and the effect of mTOR inhibition on CNV development. Results In experiment A, we detected high levels of p-mTOR S2448 and p-mTOR S2481 from the 5th to 12th day of laser injury. Immunofluorescence imaging of cryosections of mice sacrificed on day 7 revealed greater co-immunoreactivity of p-mTOR S2448 positive cells with CD11b and F4/80, while p-mTOR S2481 positive cells showed colocalization with CD31, α-SMA, and cytokeratin. In experiment B, rapamycin injection during the active period of mTOR signaling demonstrated near-complete inhibition of CNV lesion as well as significant induction of autophagy. Conclusion Our study suggests the mTOR as a critical player during CNV development in laser-induced mouse model through differentially acting with the mTORC1 and mTORC2. mTORC1 activity was high predominantly in inflammatory cells in CNV lesion, while mTORC2 activity was higher in vascular components and the RPE.

【 授权许可】

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