Maternal haploids are preferentially induced by CENH3-tailswap transgenic complementation in maize

Frontiers in Plant Science

Barry eMartin¹  Dakota eStarr²  Jamie eMcCuiston²  Wenling eWang²  Timothy eKelliher²  Heng eZhong²  Michael L. Nuccio² 

[1] Cibo Technologies;Syngenta Crop Protection;

关键词: RNA Interference;    centromeres;    doubled haploids;    haploid induction;    CENH3;    Mutant complementation;   

DOI  :  10.3389/fpls.2016.00414

来源: DOAJ

【 摘 要 】

Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 -/- and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after independently controlling for inducer parent sex and transgene zygosity. CENH3 fusion proteins were localized to centromeres and did not cause vegetative defects or male sterility. CENH3:RNAi lines did not demonstrate consistent knockdown and rarely produced haploids. In contrast, many of the complemented CENH3 -/- lines produced haploids at low frequencies. The rate of gynogenic haploid induction reached a maximum of 3.6% in several hemizygous individuals when backcrossed as males. These results demonstrate that CENH3-tailswap transgenes can be used to engineer in vivo haploid induction systems into maize plants.

【 授权许可】

Unknown