Evaluation of the Saponin Content in Panax vietnamensis Acclimatized to Lam Dong Province by HPLC–UV/CAD

Molecules

Jeong Hill Park¹  Kim Long Vu-Huynh²  Huong Thuy Le²  Minh Duc Nguyen²  Huy Truong Nguyen²  Hien Minh Nguyen²  Thi Hong Van Le³ 

[1] College of Pharmacy, Seoul National University, Seoul 08826, Korea;Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam;Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam;

关键词: Panax vietnamensis;    Vietnamese ginseng;    HPLC–CAD;    majonoside;    vina-ginsenoside;    ocotillol;   

DOI  :  10.3390/molecules26175373

来源: DOAJ

【 摘 要 】

Panax vietnamensis, or Vietnamese ginseng (VG), an endemic Panax species in Vietnam, possesses a unique saponin profile and interesting biological activities. This plant is presently in danger of extinction due to over-exploitation, resulting in many preservation efforts towards the geographical acclimatization of VG. Yet, no information on the saponin content of the acclimatized VG, an important quality indicator, is available. Here, we analyzed the saponin content in the underground parts of two- to five-year-old VG plants acclimatized to Lam Dong province. Nine characteristic saponins, including notoginsenoside-R1, ginsenoside-Rg1, -Rb1, -Rd, majonoside-R1, -R2 vina-ginsenoside-R2, -R11, and pseudoginsenoside-RT4, were simultaneously determined by HPLC coupled with UV and with a charged aerosol detector (CAD). Analyzing the results illustrated that the detection of characteristic ocotillol-type saponins in VG by CAD presented a superior capacity compared with that of UV, thus implying a preferential choice of CAD for the analysis of VG. The quantitative results indicating the saponin content in the underground parts of VG showed an increasing tendency from two to five years old, with the root and the rhizome exhibiting different saponin accumulation patterns. This is the first study that reveals the preliminary success of VG acclimatization and thereby encourages the continuing efforts to develop this valuable saponin-rich plant.

【 授权许可】

Unknown