Allyl Isothiocyanate Ameliorates Dextran Sodium Sulfate-Induced Colitis in Mouse by Enhancing Tight Junction and Mucin Expression

International Journal of Molecular Sciences

MinWoo Kim¹  Seungho Choi¹  YeoSung Yoon¹  Ju-Hee Kang²  SunYeou Kim²  SeungHyun Oh² 

[1] Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, 08826 Seoul, Korea;Gachon Institute of Pharmaceutical Sciences, Gachon University, 21936 Incheon, Korea;

关键词: allyl isothiocyanate (AITC);    Wasabia japonica;    intestinal epithelial barrier;    tight junction;    mucin;    mucin 2 (MUC2);    goblet cell;    dextran sodium sulfate (DSS);    colitis;   

DOI  :  10.3390/ijms19072025

来源: DOAJ

【 摘 要 】

Inflammatory bowel disease (IBD) is characterized by chronic or recurrent inflammation of the gastrointestinal tract. Even though the current strategies to treat IBD include anti-inflammatory drugs and immune modulators, these treatments have side-effects. New strategies are, therefore, required to overcome the limitations of the therapies. In this study, we investigated the anti-colitic effects of allyl isothiocyanate (AITC), which is an active ingredient present in Wasabia japonica. The DSS-induced colitis model in the mouse was used to mimic human IBD and we observed that AITC treatment ameliorated the severity of colitis. We further studied the mechanism involved to ameliorate the colitis. To investigate the involvement of AITC on the intestinal barrier function, the effect on the intercellular tight junction was evaluated in the Caco-2 cell line while mucin expression was assessed in the LS174T cell line. AITC positively regulated tight junction proteins and mucin 2 (MUC2) against DSS-induced damage or depletion. Our data of in vivo studies were also consistent with the in vitro results. Furthermore, we observed that MUC2 increased by AITC is dependent on ERK signaling. In conclusion, we propose that AITC can be considered as a new strategy for treating IBD by modulating tight junction proteins and mucin.

【 授权许可】

Unknown