In vitro responses of Stevia rebaudiana Bert. to MS basal medium supplemented with 6-benzylaminopurine and indole-3-butyric acid

Fundamental and Applied Agriculture

Mansor Hakiman¹  Nor Asikin Asmuni² 

[1] 1Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2Laboratory of Sustainable Resources Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;

关键词: 6-benzylaminopurine;    indole-3-butyric acid;    in vitro responses;    stevia rebaudiana;   

DOI  :  10.5455/faa.103572

来源: DOAJ

【 摘 要 】

Propagation ofS. rebaudiana Bert. can be cultivated by seed germination and stem cutting however, seed germination of this plant is not efficient due to low fertility and produce a heterogeneous population, thus producing unstable sweetening level. Thus, micropropagation can overcome these problems by producing large number of clone plantlets within a short time. In this research, the objective is to study the effect of different concentrations of plant growth regulators; auxin and cytokinin on in vitro responses of S. rebaudiana Bert. The nodal segments used as explant and inoculated onto full strength of Murashige and Skoog (MS) media. The medium supplemented with five concentrations of 6-benzylaminopurine (BAP); 0, 1, 2, 3 and 4 mg/L and also indole-3-butyric acid (IBA); 0, 0.5, 1.0, 1.5 and 2.0 mg/L. After six weeks of culture, number and length of shoots, number and length of roots and callus percentage were recorded. The results showed that 3.0 mg/L BAP produced the highest number of shoots per explant and for rooting, the best treatment that can be used was 1.5 mg/L IBA without cytokinin. [Fundam Appl Agric 2020; 5(2.000): 243-247]

【 授权许可】

Unknown