Slug Feeding Triggers Dynamic Metabolomic and Transcriptomic Responses Leading to Induced Resistance in Solanum dulcamara

Frontiers in Plant Science

Heidrun Huber¹  Tobias Lortzing²  Anke Steppuhn²  Onno W. Calf⁴  Janny L. Peters⁵  Alexander Weinhold⁷  Nicole M. van Dam⁷  Yvonne Poeschl⁸ 

[1] Department of Experimental Plant Ecology, Institute for Water and Wetland Research, Radboud University, Nijmegen, Netherlands;Department of Molecular Botany, Institute of Biology, University of Hohenheim, Stuttgart, Germany;Department of Molecular Ecology, Institute of Biology, Free University of Berlin, Berlin, Germany;Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, Nijmegen, Netherlands;Department of Plant Systems Physiology, Institute for Water and Wetland Research, Radboud University, Nijmegen, Netherlands;German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany;Institute of Biodiversity, Friedrich-Schiller University of Jena, Jena, Germany;Institute of Computer Science, Martin Luther University of Halle-Wittenberg, Halle (Saale), Germany;

关键词: defense signaling;    eco-metabolomics;    LC-qToF-MS;    microarray;    plant–herbivore interaction;    secondary metabolites;   

DOI  :  10.3389/fpls.2020.00803

来源: DOAJ

【 摘 要 】

Induced plant responses to insect herbivores are well studied, but we know very little about responses to gastropod feeding. We aim to identify the temporal dynamics of signaling- and defense-related plant responses after slug feeding in relation to induced resistance. We exposed Solanum dulcamara plants to feeding by the gray field slug (GFS; Deroceras reticulatum) for different periods and tested disks of local and systemic leaves in preference assays. Induced responses were analyzed using metabolomics and transcriptomics. GFS feeding induced local and systemic responses. Slug feeding for 72 h more strongly affected the plant metabolome than 24 h feeding. It increased the levels of a glycoalkaloid (solasonine), phenolamides, anthocyanins, and trypsin protease inhibitors as well as polyphenol oxidase activity. Phytohormone and transcriptome analyses revealed that jasmonic acid, abscisic acid and salicylic acid signaling were activated. GFS feeding upregulated more genes than that it downregulated. The response directly after feeding was more than five times higher than after an additional 24 h without feeding. Our research showed that GFS, like most chewing insects, triggers anti-herbivore defenses by activating defense signaling pathways, resulting in increased resistance to further slug feeding. Slug herbivory may therefore impact other herbivores in the community.

【 授权许可】

Unknown