The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy

iScience

Aileen K. Sewell¹  Christopher C. Ebmeier¹  Zachary C. Poss¹  William M. Old¹  Jeremy R. Jacobsen¹  Min Han¹ 

[1] Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA;

关键词: Cell biology;    Functional aspects of cell biology;    Developmental biology;    Omics;    Proteomics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The protein kinase complex target of rapamycin complex 1 (TORC1) is a critical mediator of nutrient sensing that has been widely studied in cultured cells and yeast, yet our understanding of the regulatory activities of TORC1 in the context of a whole, multi-cellular organism is still very limited. Using Caenorhabditis elegans, we analyzed the DAF-15/Raptor-dependent phosphoproteome by quantitative mass spectrometry and characterized direct kinase targets by in vitro kinase assays. Here, we show new targets of TORC1 that indicate previously unknown regulation of transcription and autophagy. Our results further show that DAF-15/Raptor is differentially expressed during postembryonic development, suggesting a dynamic role for TORC1 signaling throughout the life span. This study provides a comprehensive view of the TORC1 phosphoproteome, reveals more than 100 DAF-15/Raptor-dependent phosphosites that reflect the complex function of TORC1 in a whole, multi-cellular organism, and serves as a rich resource to the field.

【 授权许可】

Unknown