Antioxidants | |
Photo-Oxidative Protection of Chlorophyll a in C-Phycocyanin Aqueous Medium | |
Bong-Geun Chung1  Seung-Yop Lee1  Jae-Hyun Lim1  Ji-Eun Hong1  Tae-Yoon Kim1  Han-Bin Oh2  Hwa-Yong Jang2  | |
[1] Department of Biomedical Engineering, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul 04107, Korea;Department of Chemistry, Sogang University, Baekbeom-ro 35, Mapo-gu, Seoul 04107, Korea; | |
关键词: chlorophyll a; antioxidant activity; photodegradation; phycocyanin; cytotoxicity; | |
DOI : 10.3390/antiox9121235 | |
来源: DOAJ |
【 摘 要 】
In this study, potential protection of chlorophyll a from illumination and oxidation-induced decomposition has been examined using C-phycocyanin (C-PC) aqueous medium. Photo-oxidation resistance of chlorophyll a was monitored in various aqueous media using ultraviolet-visible spectroscopy and direct-infusion atmospheric pressure chemical ionization mass spectrometry analysis. The spectroscopy results showed that chlorophyll a in C-PC medium experienced the lowest rate of conversion to its derivatives; thus, it was demonstrated that chlorophyll a was mostly intact in the C-PC medium. Furthermore, the C-PC treated with chlorophyll a showed the lowest concentrations of malondialdehyde, and chlorophyll a in C-PC medium did not cause serious damage to human liver cells in vitro after intensive illumination. Therefore, we propose a new method of protecting chlorophyll a from photodegradation and oxidation using C-PC aqueous medium.
【 授权许可】
Unknown