Characterization of genetic diversity on tropical Trichoderma germplasm by sequencing of rRNA internal transcribed spacers

BMC Research Notes

Yara Barros Feitosa¹  Leandro Lopes Loguercio²  Valter Cruz-Magalhães²  Ronaldo Costa Argolo-Filho²  Jorge Teodoro de Souza³ 

[1] Center of Nuclear Energy in Agriculture (CENA), State University of São Paulo (USP);Department of Biological Sciences (DCB), State University of Santa Cruz (UESC);Department of Phytopathology (DFP), Federal University of Lavras (UFLA);

关键词: Biological control;    Polymerase chain reaction;    Internal transcribed spacer;    BlastN;    Phylogeny;    Biotechnological development;   

DOI  :  10.1186/s13104-019-4694-1

来源: DOAJ

【 摘 要 】

Abstract Objective Trichoderma species are found in soil and in association with plants. They can act directly or indirectly in the biological control of plant diseases and in the promotion of plant growth, being among the most used fungi in the formulation of bioproducts applied to agricultural systems. The main objective of this study was to characterize at a first-tier level a collection of 67 Trichoderma isolates from various tropical sources, based solely on sequencing of the internal transcribed spacer (ITS) region of the rRNA genes. Our goal was to provide a preliminary idea of the baseline diversity in this collection, to combine this information later with an array of other isolate-specific physiological data. This study provides a required knowledge at molecular level for assessment of this germplasm potential as a source of biotechnological products for beneficial effects in plants. Results Sequencing of the ITS region showed that the 67 Trichoderma isolates belonged in 11 species: T. asperellum, T. atroviride, T. brevicompactum, T. harzianum, T. koningiopsis, T. longibrachiatum, T. pleuroticola, T. reesei, T. spirale, T. stromaticum and T. virens. A total of 40.3% of the isolates were very closely related to each other and similar to T. harzianum. The baseline genetic diversity found indicates that the collection has different genotypes, which can be exploited further as a source of bioproducts, aiming at providing beneficial effects to plants of interest to cope with biotic and abiotic stresses.

【 授权许可】

Unknown