Magnetic Nanocomposite Hydrogels for Directing Myofibroblast Activity in Adipose‐Derived Stem Cells

Advanced NanoBiomed Research

Jake Ireland¹  Kristopher A. Kilian²  Thomas G. Molley²  Md Shariful Islam²  Jamie J. Kruzic³ 

[1] School of Chemistry Australian Centre for Nanomedicine The University of New South Wales Sydney NSW 2052 Australia;School of Materials Science and Engineering The University of New South Wales Sydney NSW 2052 Australia;School of Mechanical and Manufacturing Engineering The University of New South Wales Sydney NSW 2052 Australia;

关键词: adipose-derived stromal cells;    differentiation;    hydrogels;    magnetoactive;    nanoparticle;    smooth muscles;   

DOI  :  10.1002/anbr.202000072

来源: DOAJ

【 摘 要 】

Dynamic cell‐culture materials that can change mechanical properties in response to extrinsic stimuli are emerging as promising tools for cell and tissue engineering research. However, most of these techniques involve a one‐way stiffening or softening through changes in the materials chemistry, which does not allow reversibility. Here, the incorporation of superparamagnetic iron‐oxide nanoparticles within poly(ethylene glycol) hydrogels as dynamic cell culture materials is demonstrated. Using simple permanent magnets and adipose‐derived stem cells, a near twofold increase in cell spread area and an accompanying 20% enrichment in cells expressing alpha‐smooth muscle actin is seen. This platform provides a means to study relationships between dynamic stiffening and cell behavior, using permanent magnets and clinically viable composite materials, with scope for use as a tool to enrich the myofibroblast population in stromal cells.

【 授权许可】

Unknown