Reduction of Deuterium Level Supports Resistance of Neurons to Glucose Deprivation and Hypoxia: Study in Cultures of Neurons and on Animals

Molecules

Arkady Moiseev¹  Alexandr Basov²  Mikhail Baryshev²  Anna Elkina²  Alexandr Kravtsov²  Stepan Dzhimak²  Stanislav Kozin²  Elena Butina³  Vadim Malyshko⁴ 

[1] Department of Organization and Support of Scientific Activities, Kuban State Agrarian University, 350044 Krasnodar, Russia;Department of Radiophysics and Nanothechnology, Physics Faculty, Kuban State University, 350040 Krasnodar, Russia;Department of Technology of Fats, Cosmetics, Commodity Science, Processes and Devices, Kuban State Technological University, 350072 Krasnodar, Russia;South Scientific Center of the Russian Academy of Sciences, Laboratory of Problems of Stable Isotope Spreading in Living Systems, 344006 Rostov-on-Don, Russia;

关键词: deuterium depleted water;    hypoxia;    neuron;    rats;   

DOI  :  10.3390/molecules27010243

来源: DOAJ

【 摘 要 】

The effect of a reduced deuterium (D) content in the incubation medium on the survival of cultured neurons in vitro and under glucose deprivation was studied. In addition, we studied the effect of a decrease in the deuterium content in the rat brain on oxidative processes in the nervous tissue, its antioxidant protection, and training of rats in the T-shaped maze test under hypoxic conditions. For experiments with cultures of neurons, 7–8-day cultures of cerebellar neurons were used. Determination of the rate of neuronal death in cultures was carried out using propidium iodide. Acute hypoxia with hypercapnia was simulated in rats by placing them in sealed vessels with a capacity of 1 L. The effect on oxidative processes in brain tissues was assessed by changes in the level of free radical oxidation and malondialdehyde. The effect on the antioxidant system of the brain was assessed by the activity of catalase. The study in the T-maze was carried out in accordance with the generally accepted methodology, the skill of alternating right-sided and left-sided loops on positive reinforcement was developed. This work has shown that a decrease in the deuterium content in the incubation medium to a level of −357‰ has a neuroprotective effect, increasing the survival rate of cultured neurons under glucose deprivation. When exposed to hypoxia, a preliminary decrease in the deuterium content in the rat brain to −261‰ prevents the development of oxidative stress in their nervous tissue and preserves the learning ability of animals in the T-shaped maze test at the level of the control group. A similar protective effect during the modification of the ²H/¹H internal environment of the body by the consumption of DDW can potentially be used for the prevention of pathological conditions associated with the development of oxidative stress with damage to the central nervous system.

【 授权许可】

Unknown