Pin1 Regulates IL-5 Induced Eosinophil Polarization and Migration

Cells

Zhong-Jian Shen¹  JamesS. Malter¹  MelissaA. O’Neal¹  Jie Hu¹ 

[1] Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;

关键词: eosinophils;    asthma;    IL-5;    allergy;    Pin1;    shape;   

DOI  :  10.3390/cells10020211

来源: DOAJ

【 摘 要 】

Eosinophils become polarized in response to cytokines such IL-5 or eotaxin prior to directional migration. Polarization is preceded by F-actin assembly, but the mechanisms that regulate these events and how the shape change influences cell migration from the peripheral blood into the lung remain unclear. In this study, we show that the prolyl isomerase, Pin1, is required for IL-5-induced Eos polarization and migration. Co-immunoprecipitation and immunofluorescence analysis revealed that Pin1 directly interacts with members of Rho GTPase family. Mouse eosinophils lacking Pin1 or human cells treated with Pin1 inhibitors showed significantly reduced IL-5-induced GTPase activity and cofilin phosphorylation, resulting in reduced F-actin polymerization, cell polarization, and directional migration to chemokines. Our result suggests that Pin1 regulates cytoskeletal re-organization, eosinophil morphology, and cell migration through the modulation of Rho GTPase activity. Targeting Pin1 along with GTPases could provide a new approach to reduce pulmonary Eos accumulation during asthmatic exacerbations.

【 授权许可】

Unknown