Genomic profiling and expression analysis of the diacylglycerol kinase gene family in heterologous hexaploid wheat

PeerJ

Ke Zhang¹  Shijun Tian²  Wenjing Li²  Xuyang Si²  Yanyun Pan²  Hongyan Zhang²  Xiaowei Jia²  Yangyang Jia² 

[1] College of Agronomy, Hebei Agricultural University/State Key Laboratory of North China Crop Improvement and Regulation/Key Laboratory of Crop Growth Regulation of Hebei Province, Baoding, Hebei, China;College of Life Science, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Baoding, Hebei, China;

关键词: Abiotic stress;    Wheat;    Diacylglycerol kinase;    Gene family;   

DOI  :  10.7717/peerj.12480

来源: DOAJ

【 摘 要 】

The inositol phospholipid signaling system mediates plant growth, development, and responses to adverse conditions. Diacylglycerol kinase (DGK) is one of the key enzymes in the phosphoinositide-cycle (PI-cycle), which catalyzes the phosphorylation of diacylglycerol (DAG) to form phosphatidic acid (PA). To date, comprehensive genomic and functional analyses of DGKs have not been reported in wheat. In this study, 24 DGK gene family members from the wheat genome (TaDGKs) were identified and analyzed. Each putative protein was found to consist of a DGK catalytic domain and an accessory domain. The analyses of phylogenetic and gene structure analyses revealed that each TaDGK gene could be grouped into clusters I, II, or III. In each phylogenetic subgroup, the TaDGKs demonstrated high conservation of functional domains, for example, of gene structure and amino acid sequences. Four coding sequences were then cloned from Chinese Spring wheat. Expression analysis of these four genes revealed that each had a unique spatial and developmental expression pattern, indicating their functional diversification across wheat growth and development processes. Additionally, TaDGKs were also prominently up-regulated under salt and drought stresses, suggesting their possible roles in dealing with adverse environmental conditions. Further cis-regulatory elements analysis elucidated transcriptional regulation and potential biological functions. These results provide valuable information for understanding the putative functions of DGKs in wheat and support deeper functional analysis of this pivotal gene family. The 24 TaDGKs identified and analyzed in this study provide a strong foundation for further exploration of the biological function and regulatory mechanisms of TaDGKs in response to environmental stimuli.

【 授权许可】

Unknown