Verification and application of a modified carbapenem inactivation method (mCIM) on Pseudomonas aeruginosa: a potential screening methodology on carbapenemases phenotype in Bacillus cereus

Bioengineered

Xiaoyi Fan¹  Juzhen Liu¹  Yunzu Huang¹  Guangchao Yu¹  Junyan Liu²  Congxiu Ye³  Juexin Wang⁴  Caiying Bai⁵  Thanapop Soteyome⁶  Birthe V. Kjellerup⁷  Xin Lin⁸  Liyan Liu⁸  Xiaoxi Bai⁸  Zhenbo Xu⁸ 

[1]Clinical Laboratory Center, The First Affiliated Hospital of Jinan University, Guangzhou, PR China

[2]College of Light Industry and Food Science, Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Academy of Contemporary Agricultural Engineering Innovations, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, China

[3]Department of Dermatology and Venerology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China

[4]Ganzhou Center for Disease Control and Prevention, Ganzhou, PR China

[5]Guangdong Women and Children Hospital, Guangzhou, Guangdong, China

[6]Home Economics Technology, Rajamangala University of Technology Phra Nakhon, Bangkok, Thailand

[7]Research Institute for Food Nutrition and Human Health, Guangzhou, Guangdong, China

[8]School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, South China University of Technology, Guangzhou, Guangdong, China

关键词: P. aeruginosa;    CRPA;    mCIM;    antibiotic;    carbapenemase phenotypes screening;    drug resistance;   

DOI  :  10.1080/21655979.2022.2072601

来源: DOAJ

【 摘 要 】

Antimicrobial resistance (AMR) has been a leading issue for human health globally threatening the achievement of several of the Sustainable Development Goals (SDGs). Originated from Bacillus cereus, carbapenemases phenotype has been considered to be a major concern in AMR. In this study, the AMR identification rate of P. aeruginosa isolates and infections in FAHJU showed an obvious upward trend from 2012 to 2016. All 88 carbapenem-resistant P. aeruginosa strains were screened for carbapenemase phenotype by modified Carbapenem Inactivation Method (mCIM), and these results of mCIM were compared with traditional PCR results. The isolates of P. aeruginosa and infected patients showed obvious upward trend from 2012 to 2016. The drug resistance to common clinical antibiotics was serious that the clinical rational use of antibiotics should be strengthened, which is in accordance with the Global Antimicrobial Resistance and Use Surveillance System (GLASS) report. In comparison, the results of mCIM showed that 18 out of 88 CRPA strains were carbapenemase positive, which were completely consistent with the results yielded by PCR method. Therefore, it is convinced that this mCIM methodology is a simple and quick method for detected carbapenemases producing P. aeruginosa and has a potential capability in carbapenemases phenotype of pathogen like B. cereus, which will undoubtedly aid in the AMR therapy.

【 授权许可】

Unknown